脐血源神经干细胞移植治疗陈旧性脊髓损伤
Umbilical cord blood neural stem cells for obsolete spinal cord injury
摘要 背景:随着医学的进步,脊髓损伤患者的预后未得到明显的改善,大部分患者常遗留严重的并发症。如今,许多实验室和临床研究成果已经提示细胞治疗具有巨大潜能,特别是脐血干细胞在神经系统疾病中的应用。目的:观察脐血源神经干细胞治疗陈旧性脊髓损伤的可行性和临床疗效。方法:无菌条件下采集新生儿脐血,在体外条件下分化成神经干细胞,制成细胞浓度为109 L-1的细胞悬液,经腰椎穿刺(L3-L4或L4-L5)注入蛛网膜下腔3 mL。移植前和移植后3个月对脊髓损伤患者行脊髓损伤神经学分类国际标准(ASIA)评分和膀胱残余尿量测定。结果与结论:脐血源神经干细胞移植后患者生命体征平稳,移植后3个月 ASIA 各项评分较前提高、膀胱残余尿量减少,差异有显著性意义(P<0.05)。可见脐血源神经干细胞移植作为一种新的治疗方法,可以改善陈旧性脊髓损伤患者肢体功能,提高患者生活质量。
Abstract:
BACKGROUND:With the medical development, prognostic outcomes of spinal cord injuries have not been improved significantly, and most patients also suffer from severe complications. Nowadays, lots of laboratories and clinical researches have suggested that celltherapy has a great potential, especial y the application of umbilical cord blood stem cells in nervous system diseases. OBJECTIVE:To explore the feasibility and clinical effect of umbilical cord blood neural stem cells transplantation for patients with obsolete spinal cord injury. METHODS:Umbilical cord blood was harvested from newborns under aseptic condition, and differentiated into neural stem cells in vitro that were prepared into cellsuspension at a concentration of 109/L. The cellsuspension (3 mL) was injected via the L 3-4 or L 4-5 into the subarachnoid space. American Spinal Injury Association (ASIA) scores and the residual urine were assessed before and 3 months after transplantation. RESULTS AND CONCLUSION:After transplantation, al the patients showed a stable life indication. Three months later, ASIA scores were increased and the residual urine decreased, which significantly differed from those before transplantation (P<0.05). These findings indicate that umbilical cord blood neural stem cells transplantation is a new treatment that can improve the limb function and life quality of patients with obsolete spinal cord injury.
Author: Wu Yue-kui Wang Shang-wu Ma Jian-hua Yi Bo Gao Bing-bing Qin Jia-zhen Yang Zhi-jun Dai Yi-wu
作者单位: 首都医科大学密云教学医院神经外科,北京市,101500 解放军北京军区总医院附属八一脑科医院,北京市,100700
刊 名 中国组织工程研究 ISTICPKU
年,卷(期): 2014, (41)
分类号: R394.2
机标分类号: R45 R25
在线出版日期: 2014年11月26日
基金项目: 国家自然科学基金