雷公藤多糖诱导胰腺干细胞成胰岛样细胞团
Tripterygium wilfordii polysaccharide induces differentiation of pancreatic stem cells into islet-like cell clusters
摘要 背景:胰岛细胞来源不足说明胰岛细胞移植治疗糖尿病不能满足临床需要.因此,体外诱导胰腺干细胞分化为胰岛细胞成为研究的热点.目的:观察雷公藤多糖对小鼠胰岛来源的胰腺干细胞的诱导分化作用,从而探索中药诱导胰腺干细胞分化为胰岛β细胞的作用.方法:采用雷公藤多糖体外诱导经纯化的小鼠胰腺干细胞分化为胰岛细胞.胰岛样细胞团进行形态学观察,双硫腙染色和Western blot分析.结果与结论:①细胞形态学、细胞生长特性及免疫细胞化学染色显示,实验不仅得到了纯度高的小鼠胰腺干细胞,而且该细胞经雷公藤多糖诱导后能形成球形的胰岛样结构,有细长的蒂与培养瓶底部相连,双硫腙染色呈铁红色;②Western-blot检测显示,胰岛样细胞团中有β细胞素蛋白的表达;③结果表明,小鼠胰腺干细胞可以体外经雷公藤多糖诱导分化为含有β细胞的胰岛样细胞团.
Abstract:
BACKGROUND: Inadequate sources of islet cells mean that islet cell transplantation for diabetes cannot meet the clinical demand.Therefore,in vitro induction of pancreatic stem cells to differentiate into islets has become a focus of research. OBJECTIVE:To study the effect of Tripterygium wilfordii polysaccharides on the differentiation of pancreatic stem cells from islets in mice, so as to explore the effect of traditional Chinese medicine on the differentiation of pancreatic stem cells into pancreatic beta cells. METHODS:Tripterygium wilfordii polysaccharide was used to induce the differentiation of purified mouse pancreatic stem cells into islets in vitro.The islet-like cell clusters then underwent morphologic observation, dithizone (DTZ) staining, and western blot analysis. RESULTS AND CONCLUSION: Cell morphology, cell growth characteristics and immunocytochemical staining showed that mouse pancreatic stem cells were obtained.They were induced by Tripterygium wilfordii polysaccharide into spherical islet-like structures, which had a spindly pedicle connected with the bottom of the culture flask, and were DTZ-stained to iron red. Western blot assay detected β-cytokine proteins in the islet-like cell clusters. These findings confirm that mouse pancreatic stem cells can be induced to differentiate into islet-like cell clusters containing β cells in vitro by Tripterygium wilfordii polysaccharide.
Author: Cen Yan-hui Li Zhong-hua Jia Wei Yang Rui Bao Juan He Guo-zhen Wu Xiao-jun Zhong Jing Deng Hui-feng Shi Lei
作者单位: 广西中医药大学,广西壮族自治区南宁市 530222;广西壮族自治区中医药大学第一附属医院,广西壮族自治区南宁市 530023 广西中医药大学,广西壮族自治区南宁市,530222
刊 名 中国组织工程研究 ISTICPKU
年,卷(期): 2018, 22(5)
分类号: R394.2
在线出版日期: 2018年4月3日
基金项目: 国家自然科学基金项目,中国博士后科学基金,广西壮族自治区卫生厅中医药科技专项课题,广西自然科学基金(2014GXNSFAA118177)the National Natural Science Foundation of China,No.81503406,the China Postdoctoral Science Foundation,No.2017M6232,the Special Fund for Traditional Chinese Medicine by the Health Department of Guangxi Zhuang Autonomous Region,No.GZBZ16-07,GZLC16-23,the Natural Science Foundation of Guangxi Zhuang Autonomous Region,No.2014GXNSFAA118177