成人骨髓间质干细胞定向诱导为神经元样细胞的研究
Human mesenchymal stem cells differentiate into neuron-like cells
摘要 目的:体外定向诱导成人骨髓间质干细胞(MSC)分化为神经元样细胞。方法:采用Ficoll-Paque液(1.077×103g/L)离心分离成人MSC,体外扩增,分别采用含巯基乙醇和硫代甘油等试剂的无血清DMEM诱导MSC分化为神经元。免疫组化鉴定神经元烯醇化酶(NSE)、神经丝蛋白(NF)、胶质纤维酸性蛋白(GFAP)的表达。结果:成人骨髓间质干细胞在体外扩增5代可获5×107个细胞。加入巯基乙醇等或硫代甘油等诱导剂诱导后,MSC胞体收缩,突起伸出;免疫组化显示诱导出的神经元样细胞NSE、NF表达阳性,GFAP阴性。结论:成人骨髓间质干细胞在体外可以分化为神经元样细胞。
Abstract:
AIM:To investigate the differentiation from human mesenchymal stem cells(hMSC) into neuron-like cells.METHODS:hMSC were separated from rib marrow with Ficoll-Paque reagent and expanded in culture medium. hMSC were induced to differentiate with DMEM/monothioglycerol or DMEM/β-mercaptoethanol, respectively. Neuron-specific enolase(NSE), neurofilament(NF), and glial fibrillary acidic protein(GFAP) were detected by immunohistochemistry. RESULTS:hMSC were expanded as undifferentiated cells in culture for more than 5 passages. When treated with monothioglycerol or β-mercaptoethanol for 5 hours, hMSC exhibited neuronal phenotype. The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION:hMSC can be induced to differentiate into neurous.
Author: XIANG Peng XIA Wen-jie Zhang Xiu-ming LI Yan LI Shu-nong ZHANG Li-rong CHEN Zhen-guang
作者单位: 中山医科大学病理生理教研室, 广州药学院病理生理教研室, 中山医科大学附属第一医院心胸外科,
刊 名 中国病理生理杂志 ISTICPKU
年,卷(期): 2001, 17(5)
分类号: R338.8
机标分类号: R68 R6
在线出版日期: 2004年1月8日
基金项目: 国家重点基础研究发展计划(973计划)