Isolation, Culture and Induced Differentiation of Fetal Porcine Islet Derived Pancreatic Stem Cell
Abstract:
To isolate and culture the porcine pancreatic stem cells and investigate their function, the fetal porcine pancreatic stem cells were isolated by the method of suspending plus adhering culture. The isolated cells were then identified by irnmunohistochemical staining, and their culture viability measured through the MTT method in vitro. This induced them to differentiate into endocrine cells and detect their function. The isolated IPSCS did not express nestin, but expressed CK-19, a marker of ductal epithelia cells and oc-actin, a smooth muscle marker, demonstrating the growth characteristics of ES-like cells, and strong proliferative ability, after 18 passages. They could excrete insulin, and showed ultrastructure changes after being induced. Porcine pancreatic stem cells can be isolated by this method, induced to form islet-like clusters, and can secret insulin.
Author: FENG Ruo-peng ZHANG Hui-ru WANG Yun QIAO Hai ZHAO Ting SHEN Wen-zheng DOU Zhong-ying
作者单位: Shaanxi Center of National Stem Cell Engineering & Technology Research Center, Northwest A & F University, Yangling 712100, P.R.China Shaanxi Center of National Stem Cell Engineering & Technology Research Center, Northwest A & F University, Yangling 712100, P.R.China;Biological Engineering College, Henan Industrial University, Zhengzhou 450052, P.R.China
年,卷(期): 2007, 6(6)
分类号: S8
机标分类号: R96 R54
在线出版日期: 2007年7月16日
基金项目: 国家高技术研究发展计划(863计划),the Key Program of Ministry of Education of China