空心菜浮床对鱼塘水质和微生物多样性的影响
Influence on water quality andmicrobial diversity in fish pond byIpomoea aquatica floating-bed
摘要 为从环境微生物的角度探讨生物浮床的水质调节机理,采用宏基因组学测序技术和生物信息学手段,分析了环境微生物和根系微生物群落的多样性.研究结果表明,鱼菜共生模式下优势菌门是变形菌门(Proteobacteria)、蓝细菌门(Cyanobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)和厚壁菌门(Firmicutes)等,主要菌属是聚球藻属(Synechococcus)、hgcI_clade、CL500-29_marine_group、芽孢杆菌属(Bacillus)和分支杆菌属(Mycobacterium)等;空心菜根系微生物群落中最丰富的优势菌门是变形菌门和蓝细菌门,主要菌属是红细菌属(Rhodobacter)、噬氢菌属(Hydrogenophaga)和聚球藻属等.研究结果还表明低覆盖率(3.5%)的空心菜生物浮床能够增加水体的溶解氧含量,降低水体中含氮化合物的浓度和改善养殖环境中的微生态平衡,提高有益菌和氮循环细菌的含量.
Abstract:
In order to investigate the influence on water quality and microbial diversity in intensive fish pond byIpomoea aquatica floating-bed, and the composition and diversity of microbial communities in the samples were researched based on high throughput sequencing of 16S rRNA gene. The results revealed that the dominant bacteria were phyla Proteobacteria, Cyanobacteria, Bacteroidetes, Actinobacteria, Chloroflexi and Firmicutes, the dominant bacteria were genera Synechococcus,hgcI_clade,CL500-29_marine_group,Bacillus andMycobacterium in the Aquaponics System, and the most dominant bacteria of rhizosphere microorganisms were phyla Proteobacteria and Cyanobacteria, the dominant bacteria were genera Rhodobacter,Hydrogenophaga andSynechococcus. Moreover, the results also showed that low coverage (3.5%) ofIpomoea aquatica floating-bed increased the dissolved oxygen content and reduced the concentration of nitrogen compounds, and improved micro-ecological balance and increased the content of the beneficial bacteria and nitrogen cycle bacteria in aquaculture water body.
Author: LI Jian-zhu HOU Jie ZHANG Peng-fei LIU Yao-quan XIA Run-hong MA Xu-fa
作者单位: 华中农业大学,湖北武汉,430070
刊 名 中国环境科学 ISTICEIPKU
年,卷(期): 2016, 36(10)
分类号: X171.1 S917.1
机标分类号: X53 S63
在线出版日期: 2016年11月18日
基金项目: 国家科技支撑计划课题,湖北省自然科学基金