青藏高原盐湖细菌群落与超盐环境因素的相关性
Correlation analysis of bacterial community and hypersaline environmental factors in extreme salt lakes on the Qinghai-Tibet Plateau
摘要 以青藏高原极端盐湖为研究对象,采用16S rRNA基因(V3~V4区)高通量测序方法分析盐湖细菌的群落结构差异和超盐环境因子的制约相关性.结果显示,超盐环境样本中细菌的群落结构组成相对稳定,进化趋同,且复杂度和多样性指数显著低于非超盐环境.青藏高原超盐环境中细菌的优势类群是厚壁菌门Firmicutes(74.04%~81.04%)、次为变形菌门Proteobacteria(15.51%~20.06%)、拟杆菌门Bacteroidetes(2.68%~4.84%)和放线菌门Actinobacteria(0.71%~2.45%).比较分析其它类型超盐环境,青藏高原超盐环境具有高丰度的特色属群芽孢杆菌属Bacillus(50.63%~58.35%)、乳球杆菌属Lactococcus(9.28%~11.81%)和海洋芽孢杆菌属Oceanobacillus(8.41%~10.52%).基于典范对应CCA分析,表明优势属群(Bacillus、Lactococcus、Pseudomonas、Oceanobacillus、Stenotrophomonas、Psychrobacter、Myroides、Brochothrix和Arthrobacter)和超盐样本的聚集,与环境因子(总盐度、Cl-、K+、Mg2+和CO32-浓度)呈明显的正相关.
Abstract:
The bacterial structure and diversity of extremely salty lakes on the Qinghai-Tibet Plateau were investigated using high-throughput sequencing approach based on 16S rRNA gene (V3~V4region), and then the difference of community compositions and the restrict correlation of hypersaline environmental factors were investigated. Results showed that the bacterial communities of hypersaline samples were relatively stable and evolutionally convergent, and the complexity and the diversity index were significantly lower than the usual salt environment. The dominant bacterial phyla were affiliated to Firmicutes (74.04% to 81.04% of relative abundance proportion), followed by Proteobacteria (15.51% to 20.06%), Bacteroidetes (2.68% to 4.84%) and Actinobacteria (0.71% to 2.45%). Base on the comparative analysis of other types of hypersaline lakes, the characteristic genera in this study were Bacillus(50.63% to 58.35%),Lactococcus(9.28% to 11.81%) and Oceanobacillus(8.41% to 10.52%). Canonical correspondence analysis showed that the dominant bacterial genera (including Bacillus,Lactococcus,Pseudomonas,Oceanobacillus,Stenotrophomonas,Psychrobacter, Myroides,Brochothrix and Arthrobacter) and the hypersaline samples cluster was positively correlated with the saline environment factors including total salinity, Cl-, K+, Mg2+, and CO32-.
Author: ZHU De-rui HAN Rui SHI Qing SHEN Guo-ping LONG Qi-fu SHUANG Jie
作者单位: 青海大学医学院,基础医学研究中心,青海 西宁 810016 青海大学农林科学院,青海省蔬菜遗传与生理重点实验室,青海西宁 810016
刊 名 中国环境科学 ISTICEIPKU
年,卷(期): 2017, 37(12)
分类号: X171.1
在线出版日期: 2018年1月10日
基金项目: 国家自然科学基金资助项目,青海省自然科学基金资助项目