MATERIALS AND METHODS FOR TREATING DISORDERS OF THE EAR

The invention is directed to a method of changing the sensory perception of an animal. The method comprises administering an expression vector comprising a nucleic acid sequence encoding an atonal-associated factor, which is expressed to produce the atonal-associated factor resulting in generation of hair cells that allow perception of stimuli in the inner ear. Also provided is a method of generating a hair cell in differentiated sensory epithelia in vivo. The method comprises contacting differentiated sensory epithelial cells with an adenoviral vector (a) deficient in one or more replication-essential gene functions of the E1 region and E4 region, (b) comprising a spacer in the E4 region, and (c) comprising a nucleic acid sequence encoding an atonal-associated factor. The nucleic acid sequence is expressed to produce the atonal-associated factor such that a hair cell is generated. An adenoviral vector encoding an atonal-associated factor also is provided.

申请(专利)号: EP20140177029
申请日期: 2004年2月19日
公开(公告)日: 2015年1月14日
公开(公告)号: EP2824185(A1)
主分类号: C12N15/86,C,C12,C12N,C12N15
分类号: C12N15/86,A61K38/00,A61K48/00,A61P27/00,C12N15/861,C,A,C12,A61,C12N,A61K,A61P,C12N15,A61K38,A61K48,A61P27
申请(专利权)人: GENVEC, INC.
发明(设计)人: BROUGH, Douglas E.