To select the appropriate tissue culture medium formulations for the subsequent propagation stage of Dendrobium Oriental Magic'Carmen'×Dendrobium'Snow Jade'hybrid combination,using the sterile tissue culture seedlings of this hybrid combination as the experimental material,5 different treatments were set up on the 1/2 MS basal medium,including different concentrations of naphthalene acetic acid(NAA)and the addition amounts of banana puree(BJ)(T1,1/2 MS+20 g/L sucrose+9 g/L agar+1 g/L activated carbon;T2,1/2 MS+20 g/L sucrose+100 g/L BJ+9 g/L agar+1 g/L activated carbon;T3,1/2 MS+20 g/L sucrose+NAA 0.5 mg/L+50 g/L BJ+9 g/L agar+1 g/L activated carbon;T4,1/2 MS+20 g/L sucrose+NAA 1.0 mg/L+100 g/L BJ+9 g/L agar+1 g/L activated carbon;T5,1/2 MS+20 g/L sucrose+NAA 2.0 mg/L+200 g/L BJ+9 g/L agar+1 g/L activated carbon).The height,number of leaves,number of roots,root length and single plant weight of the tissue culture seedlings were measured after 120 days of cultivation under different culture media.The results showed that the T5 treatment had the best overall growth performance,with the highest plant height(4.58 cm),root length(3.58 cm),and single plant weight(205.0 mg);the root length of the subculture seedlings of the T4 treatment(1.20 cm)was at a moderately high level,while the plant height(2.40 cm),leaf number(3.40),and root number(2.60)were all lower than those of the T5 and T3 treatments.Overall,the optimal medium formulation for the subculture cultivation of the hybrid combination Dendrobium Oriental Magic'Carmen'×Dendrobium'Snow Jade'is T5(1/2 MS+sucrose 20 g/L+NAA 2.0 mg/L+BJ 200 g/L+agar 9 g/L+activated carbon 1 g/L).