Effects of tissue culture medium on seed germination and seedling growth of Dendrobium Oriental Magic Carmen×Snow Jade hybrid combination
摘要
为优化杂交石斛无菌播种阶段组培培养基配方,以卡门×雪玉石斛杂交组合成熟蒴果为材料,以1/2 MS为基础培养基,设置不同浓度萘乙酸(NAA)(0、0.5、1.0、2.0 mg/L)、香蕉泥(BJ)(0、50、100、200 g/L)及琼脂(6、9 g/L)组合的10种处理,比较各处理对种子萌发率及幼苗生长状态的影响.结果表明,各处理间萌发率差异明显,变化范围在43.80%～99.07%,其中T4(NAA 1.0 mg/L+BJ 100 g/L+琼脂9 g/L)萌发率最高(99.07%);播种后90 d,T3与T4综合评分均为10分,幼苗生长健壮、叶色翠绿、分布密集.NAA与BJ协同作用表现为低浓度促进、高浓度抑制,最适组合为NAA 1.0 mg/L+BJ 100 g/L+琼脂9 g/L.本研究确定T4为最优培养基配方,可作为该杂交组合规模化无菌播种快繁的参考方案.
To optimize the tissue culture medium formulation for the aseptic sowing of hybrid Dendrobium,mature capsules of Dendrobium Oriental Magic Carmen×Snow Jade hybrid combination were used as explants.Ten treatments were designed based on 1/2 MS basal medium supplemented with different concentrations of NAA(0,0.5,1.0,2.0 mg/L),banana homogenate(BJ)(0,50,100,200 g/L),and agar(6,9 g/L).The effects of these treatments on seed germination rate and seedling growth were compared.The results showed that germination rates differed significantly among treatments,ranging from 43.80%to 99.07%.Treatment T4(NAA 1.0 mg/L+BJ 100 g/L+agar 9 g/L)gave the highest germination rate(99.07%).At 90 days after sowing,both T3 and T4 achieved a comprehensive score of 10,with vigorous seedlings,dark green leaves,and dense distribution.The synergistic effect of NAA and BJ exhibited a promotion at lower concentrations but inhibition at higher concentrations,with the optimal combination being NAA 1.0 mg/L+BJ 100 g/L+agar 9 g/L.This study identified T4 as the optimal medium formulation,which could serve as a reference for large-scale aseptic sowing and rapid propagation of this hybrid.
