Silk Sericin-inspired Poly-β-homoserine for Protein Protection
摘要
Protein stability is a critical factor that limits its application in biopharmaceuticals,clinical diagnostics,and industrial production.The inherent instability of proteins renders them susceptible to loss of activity and function under external environmental stresses,thus necessitat-ing the development of novel stabilizers to improve protein stability.Inspired by sericin,we developed heterochiral poly-β-homoserine(β-HS)that combines resistance to enzymatic degradation,straightforward synthesis,and precise composition control,while exhibiting favorable in vit-ro safety profile.The β-HS exhibits remarkable stabilizing effects on horseradish peroxidase(HRP)and β-galactosidase(β-Gal)when subjected to elevated temperature and lyophilization,respectively.Our research indicates that β-HS stabilizes proteins by assisting in the maintenance of their conformation and preventing aggregation.Additionally,β-HS demonstrates stabilizing effects on proteins with diverse physicochemical proper-ties.Therefore,this study suggests that the β-HS is a promising candidate for enhancing protein stability.
年，卷(期)：
2026, 44(9)
机标分类号：
S
Q945.78
TP332
在线出版日期：
2026年10月16日
