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动物疾病（英文）
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2026年2期
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A high-content screening platform for antiviral drug discovery using a recombinant African swine fever virus
A high-content screening platform for antiviral drug discovery using a recombinant African swine fever virus
摘要
African swine fever(ASF),caused by African swine fever virus(ASFV),is a devastating disease of domestic pigs with mortality rates approaching 100％,leading to severe global economic losses.No effective vaccines or antivi-rals are available,highlighting the urgent need for novel therapeutic strategies and efficient screening tools.We developed a recombinant ASFV-expressing dual reporter(mCherry and NanoLuc),rASFV_mChNluc,and established a high-content screening(HCS)platform optimized for cost-effective,low-labor analysis via the mCherry reporter.The assay demonstrated excellent robustness(Z'-factor=0.669±0.064)and successfully verified the activity of the known ASFV inhibitor AraC,confirming inhibition at the postinfection stage.Screening of an in-house fungal extract library(493 extracts)identified 25 hits(5.07％)that reduced viral infectivity to＜5％.Extracts from the insect fungi Beau-veria neobassiana and Samsoniella aurantia showed potent activity,with an SI＞62.81(EC50=1.99±0.71 μg/mL)and an SI＞42.92(EC50=11.65±2.99 μg/mL),respectively.Time-of-addition assays indicated that B.neobassiana acts at multiple replication stages,whereas S.aurantia targets the postinfection stage.This study establishes a robust ASFV HCS platform for efficient high-throughput antiviral discovery and highlights fungi as a promising source of novel ASFV inhibitors.
年，卷(期)：
2026, 6(2)
机标分类号：
TP393
G250
J93
在线出版日期：
2026年8月19日
