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分析检测（英文）
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2024年3期
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Rapid and Sensitive Biosensing of Salmonella Using Mechanical Step Rotation and Gold@platinum Nanozymatic Amplification
Rapid and Sensitive Biosensing of Salmonella Using Mechanical Step Rotation and Gold@platinum Nanozymatic Amplification
摘要
Inspired by ballpoint pen,an optical biosensor is developed for detection of Salmonella using a mechanical step rotator with a dropper to greatly simplify operation,a high gradient magnetic field with immune magnetic nanobeads to specifi-cally separate bacteria,and immune gold@platinum nanozymes with a smartphone to sensitively detect bacteria.First,the preloaded bacterial sample is drawn by the dropper to react with the immune magnetic nanobeads through repeated squeez-ing and releasing of the dropper,and the rotator is successively rotated and the dropper is repeatedly squeezed and released to achieve mixing of the bacteria,magnetic nanobeads and gold@platinum nanozymes,followed by magnetic separation to remove the background.Then,the conjugates are effectively washed with skim milk and ultrapure water,and the substrate are rapidly catalyzed into the product.Finally,the blue product is photographed and analyzed using a smartphone to obtain the Salmonella concentration.This biosensor could detect Salmonella as low as 56 CFU/mL in～25 min.
Author：
Meixuan Li
Lei Wang
Ai Xu
Ying Ding
Fengzhen Yang
Yanbin Li
Jianhan Lin
作者单位：
Key Laboratory of Agricultural Information Acquisition Technology,Ministry of Agriculture and Rural Affairs,China Agricultural University,Beijing 100083,China
Center of Excellence for Poultry Science,University of Arkansas,Fayetteville AR72701,USA
年，卷(期)：
2024, 8(3)
机标分类号：
TP393.4
TP242
TH166
在线出版日期：
2024年11月27日
