Fe3O4@SiO2-Protein A-oHSV/CD63 Ab for Capturing Virus and Exosomes
摘要
Protein A modified magnetic spheres(Fe3O4@SiO2-Protein A)with 22.7 emu/g saturation magnetization were prepared and characterized.These spheres had a diameter of 10 nm and was stable up to 229.2℃,and can bond 19.694 mg/g of Protein A.The Fe3O4@SiO2-Protein A was firstly combined with Ohsv-BJ-2-A antibody to capture Ohsv from disease venom,and the amount of binding virus was 1.92×10 7 to 3.44×10 7 PFU/g.Fe3O4@SiO2-Protein A was modified with CD63 antibody,which was further fixed using dissuccinimide octylate(DSS)as a crosslinking agent.The prepared Fe3O4@SiO2-Protein A-CD63 Ab was useful in isolating and enriching exosomes from cell supernatant.Compared with commercial kits,Fe3O4@SiO2-Protein A-CD63 Ab demonstrated a better purification effect,which was successfully monitored using capillary electrophoresis.The total content of exosomes protein extracted using Fe3O4@SiO2-Protein A-CD63 was 3.4 mg/g,which was obviously higher than that reported in some studies.In addition,Fe3O4@SiO2-Protein A-CD63 Ab also showed its repeatability through bonding and elution of 5 cycles,which was effective in cost saving.These indicated Fe3O4@SiO2-Protein A-CD63 Ab had the potential for large-scale purification of exosomes in practical applications.
Author：
Wenqing Zheng
Pingyi Zheng
Ran Zhao
Xinyu Xu
Xiao Zhang
Xiaoqian Yuan
Ying Xu
Zichuan Liu
Youxin Li
作者单位：
Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency,Collaborative Innovation Center of Chemical Science and Engineering,School of Pharmaceutical Science and Technology,Faculty of Medicine,Tianjin University,Tianjin 300072,China
年，卷(期)：
2024, 8(3)
机标分类号：
R730.51
R392
S
在线出版日期：
2024年11月27日
