基于杂交链式反应与双荧光协同效应的高灵敏荧光生物传感器检测卡那霉素
A sensitive fluorescence biosensor for Kanamycin detection based onhybridization chain reaction and dual-fluorophore synergy
摘要
本研究利用杂交链式反应(HCR)以及双荧光协同放大效应,设计了一种基于磁分离技术的卡那霉素高灵敏检测方法.该方法利用核酸适体特异性识别卡那霉素,释放荧光标记的互补链,触发HCR扩增,并利用SYBR Green I染料(SGI)与羧基荧光素(FAM)的荧光协同效应增强输出信号,结合氧化石墨烯(GO)淬灭背景信号,实现了卡那霉素的高灵敏度检测.该传感器检测卡那霉素的线性范围为20～300 ng/mL,检出限为5.36 ng/mL,在实际牛奶样品中的加标回收率为93.2%～109.5%.该方法具有高选择性、高灵敏度和良好的实用性,为食品中卡那霉素残留的现场检测提供了新策略.
A highly sensitive detection method for Kanamycin based on magnetic separation technology was developed by utilizing hybridization chain reaction(HCR)and dual-fluorescence signal amplification.The method employs an aptamer for specific recognition of Kanamycin,triggering HCR amplification.Signal enhancement is achieved through the synergistic fluorescence effect of SYBR Green I(SGI)and carboxyfluorescein(FAM),while background fluorescence is effectively quenched using graphene oxide(GO).The sensor exhibited a linear detection range of 20‒300 ng/mL and a limit of detection(LOD)of 5.36 ng/mL.The spiked recoveries of the method in real milk samples ranged from 93.2%to 109.5%.These results demonstrate the method's high selectivity,sensitivity,and practical utility,providing a new strategy for the on-site detection of Kanamycin residues in food.
年，卷(期)：
2026, 45(7)
分类号：
TP212
机标分类号：
TP212.3
O657.3
TB383
在线出版日期：
2026年7月30日
基金项目：
62073299:国家自然科学基金62477043:国家自然科学基金225200810071:河南省科技研发计划联合基金项目241111220400:河南省重点研发专项241111222900:河南省重点研发专项231100220800:河南省重大科技专项242102221045:河南省科技攻关项目24A535001:河南省高等学校重点科研项目，
