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2026年7期
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Two Birds with One Stone:Enzymatic Degumming and Deacidification for Arachidonic Acid-Enriched Algae Oil Using PLA1@MCM-41-C8
Two Birds with One Stone:Enzymatic Degumming and Deacidification for Arachidonic Acid-Enriched Algae Oil Using PLA1@MCM-41-C8
摘要
Algae oil contains polyunsaturated fatty acids(PUFAs)that are beneficial to human health and develop-ment.Enzymatic refining is a sustainable approach that can improve the quality and nutritional value of algae oil.However,this approach involves the use of specific enzymes to remove unwanted impurities such as phospholipids(PLs)and free fatty acids(FFAs).This study proposes a novel immobilized phos-pholipase,immobilized phospholipase A1(PLA1@MCM-41-C8),which serves a dual purpose:it is effec-tive for both degumming arachidonic acid(ARA)-enriched algal oil and its subsequent deacidification.The use of PLA1@MCM-41-C8 achieved a degumming rate of 95.9％for crude algal oil,with an ARA reten-tion rate of up to 97.3％.The acidity of the degummed oil decreased from 14.5 to 1.1 mg KOH.g-1,while simultaneously producing 29.2％high-value diacylglycerol(DAG).By comparing enzymatically degummed and deacidified ARA-enriched algae oil(EDDO)with crude ARA-enriched algae oil(CO),com-mercialized refining algae oil(CRO),it was found that enzymatic refining had minimal impact on antiox-idant stability,fatty acid composition,and oil content.In particular,the flavor characteristics of algal oil before and after enzymatic refining with PLA1@MCM-41-C8 remained unchanged and were dominated by the main volatile compounds:alcohols and aldehydes.Compared with free phospholipase A1(PLA1),PLA1@MCM-41-C8 exhibited significantly improved thermostability,pH tolerance,solvent toler-ance,and long-term reusability.These characteristics make it a promising candidate for complex oil refining in industrial applications.
年，卷(期)：
2026, 62(7)
机标分类号：
H0-05
G23
G633.41
在线出版日期：
2026年9月10日
