Comprehensive experimental design of cerium-based nanozyme for enhanced hydroxyl radicals generation
摘要
以提升跨学科的研究与探索能力为目标,设计了介孔普鲁士蓝@二氧化铈(MPB@CeO2)纳米酶的合成、光热性能及增强的羟基自由基(·OH)产生性能评价的综合性实验.采用水热法,制备了立方体结构的MPB;采用还原法,在MPB表面生长了超小尺寸的CeO2;利用红外热像仪测量了 MPB@CeO2的光热性能;以亚甲基蓝(MB)为探针,检测了 MPB@CeO2的·OH产生性能.结果表明,制备的MPB@CeO2纳米酶尺寸约为130 nm,粒径较为均一、分散性好;在1.0 W/cm2的808 nm激光照射下,浓度为100 μg/mL的MPB@CeO2在10 min内可升温37.5℃;MPB@CeO2纳米酶具有优异的·OH产生性能,且激光辐照使·OH产率提高了 15％.本实验选题前沿、实验流程完整,融合了 MPB@CeO2的合成、表征与光热/·OH产生性能评价,不仅提高了学生的动手能力和综合分析问题的能力,而且加深了对化学、材料和化学生物学等专业知识的理解和应用.
With the goal of enhancing interdisciplinary research and exploration capabilities,a compre-hensive experiment was designed,including the synthesis of mesoporous Prussian blue@cerium dioxide(MPB@CeO2)nanozymes,the evaluation of photothermal performance and the enhanced hydroxyl radi-cals(·OH)generation performance.Using hydrothermal method,MPB nanoparticles with cubic struc-ture were synthesized,and CeO2 nanoparticles with ultrasmall size were grown on the surface of MPB by a reduction method.Using an infrared thermal imager,photothermal performance of MPB@CeO2 was measured.Using methylene blue(MB)as a probe,·OH generation performance of MPB@CeO2 was detected.The results indicated that the prepared MPB@CeO2 nanozymes with a size of 130 nm have uni-form size and good dispersibility.Under the irradiation of 808 nm laser with a power density of 1.0 W/cm2,the temperature of MPB@CeO2 with 80 μg/mL increased by 37.5℃ within 10 min.MPB@CeO2 nanozymes possessed excellent·OH generation performance,and·OH yield increased by 15％by la-ser irradiation.This experiment integrated the synthesis,characterization and photothermal/·OH gener-ation performance evaluation of MPB@CeO2,which not only improved students' operation ability and comprehensive problem-solving ability,but also deepened their understanding and application of profes-sional knowledge in chemistry,materials science,and chemical biology.
