场发射扫描电镜在本科生生物实验教学中的应用
Application of fieldemission scanning electron microscopy in undergraduate biology experimental teaching
摘要
扫描电镜具有高分辨率、高放大倍数、大景深、立体感强等特点,应用领域十分广泛.扫描电镜可观察生物组织、纳米材料、机械断面等样品的表面形貌.以拟南芥为实验材料,在本科生的生物实验教学中利用扫描电镜观察拟南芥花粉粒、花粉管及雌蕊的各个结构,目的是培养学生熟练掌握扫描电镜的原理和应用、样品制备及操作流程,培养学生的动手能力、协作能力和解决问题的能力,实现扫描电镜的开放共享和精准化服务本科教学,为其他大型仪器应用于本科教学提供参考.
Scanning electron microscope(SEM)is characterized by its high resolution,high magnifi-cation,large depth of field,and strong sense of three-dimensionality,making it widely applicable in various fields.SEM can be used to observe the surface morphology of biological tissues,nanomateri-als,mechanical cross sections and other samples.In this article,Arabidopsis thaliana was used as the experimental material to enable undergraduate students to use SEM to observe the pollen grains,pollen tubes and pistils.The aim of this experimental teaching is to train students to master the principle and application of SEM,sample preparation and operation process.This hands-on experience is designed to develop students' practical skills,teamwork,and problem-solving abilities.The ultimate goal is to achieve open sharing and precise service of SEM in undergraduate teaching,providing reference for the application of other large-scale instruments in undergraduate teaching.
年，卷(期)：
2026, 29(4)
分类号：
Q-337
G642.0
机标分类号：
G7
P575.2
TB383
在线出版日期：
2026年9月24日
基金项目：
31900262:国家自然科学基金，
