首页
>
期刊首页
>
植物通讯（英文）
>
2025年12期
>
Multi-dimensional epigenomic dynamics converge on H3K4-mediated regulation of low-CO2 adaptation in Nannochloropsis oceanica
Multi-dimensional epigenomic dynamics converge on H3K4-mediated regulation of low-CO2 adaptation in Nannochloropsis oceanica
摘要
Despite their ecological and biotechnological importance,the extent to which microalgae are regulated by epigenetic mechanisms has remained poorly understood.In the model industrial microalga Nannochlor-opsis oceanica,by comprehensive,multi-dimensional epigenomic analyses,this study uncovers an epige-netic regulatory network responsive to CO2 levels.This network involves intricate interactions among DNA methylation,histone modifications,dynamic nucleosome positioning,and three-dimensional chromatin organization during adaptation to low-CO2 conditions.Although DNA methylation is minimal,histone mod-ifications-such as lysine acetylation,crotonylation,and methylation-are associated with active chro-matin states and linked to 43.1％of differentially expressed genes.Notably,histone H3K4 di-methylation(H3K4me2)displays a distinct dual-peak profile around the transcription start site and is correlated with chromatin compartment dynamics.Knockout of NO24G02310,a putative H3K4 methyltransferase gene,caused genome-wide shifts in H3K4me2 peaks and decreased H3K4me1 levels,accompanied by direct or indirect downregulation of NoHINT and NoPMA2 expression,slower algal growth,and reduced photo-synthetic efficiency(indicated by Fv/Fm),specifically under low-CO2 conditions.Deletion and overexpres-sion of genes encoding the histidine triad nucleotide-binding protein NoHINT and the plasma membrane H+-ATPase NoPMA2 confirmed their roles in growth and photosynthetic efficiency under low CO2;NoHINT influences growth and NoPMA2 affects photosynthesis.As a previously unrecognized low-CO2 adaptation mechanism,NO24G02310 likely coordinates the regulation of NoHINT and NoPMA2 through H3K4 modifi-cations.These findings provide a foundation for enhancing microalgal productivity through targeted epige-netic engineering.
年，卷(期)：
2025, (12)
机标分类号：
TP393.02
R395.5
G643
在线出版日期：
2026年4月27日
