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A method for reducing mortality resulting from viral infection, reducing viral load resulting from viral infection, or increasing immunity to viral infection in a subject is described. The method comprises administering a hybrid protein comprises a first domain comprising a sequence encoding a surface protein of an enveloped RNA virus and a second domain comprising a sequence encoding an ectodomain of a type 2 transmembrane domain protein, wherein the second domain is located at the C-terminal of the first domain. The hybrid protein or an mRNA encoding such protein can be used as a vaccine against the infection of the enveloped RNA virus.

Disclosed herein are RSV F glycoproteins and nanoparticles comprising the same, which are suitable for use in vaccines. The nanoparticles present antigens from pathogens surrounded to and associated with a detergent core resulting in enhanced stability and good immunogenicity. Dosages, formulations, and methods for preparing the vaccines and nanoparticles are also disclosed.

The present disclosure provides a triboelectric nanosensor, a screening and detecting platform, and a screening and detecting method. The triboelectric nanosensor includes a single-electrode, a solid triboelectric layer and a fusion protein. The solid triboelectric layer is coated on a surface of the single-electrode. The fusion protein includes a target protein and a histidine tag, and the fusion protein is adsorbed on a surface of the solid triboelectric layer. The screening and detecting platform includes the triboelectric nanosensor, a detection solution, a detection tank, a reaction solution, a reaction tank, and a displacement device, in which the detection solution includes a solvent and a target analyte. Thus, by taking advantage of the fast response characteristics of the triboelectric nanosensor, the screening and detecting platform can quickly detect the affinity of the interaction between the triboelectric nanosensor and the target analyte, significantly shortening the screening and detecting time.

A hybrid gamma–beta voltaic device designed for direct conversion of radiation from nuclear waste into electricity. The invention employs alternating PbO and SiC semiconductor layers reinforced with hollow copper rods acting as both electrical conductors and coolant channels. Gamma and beta radiations are absorbed and converted into charge carriers through photoelectric and betavoltaic effects. The generated charge is collected via copper channels, with heat managed through a passive coolant loop. The assembly is fully shielded, providing a long-life, maintenance-free system for nuclear waste energy recovery.

本发明涉及基因工程技术领域，提供了一种可溶高表达的三螺旋重组胶原蛋白及其制备方法。针对现有技术中胶原蛋白在原核表达体系中表达时通常存在形成包涵体、难以实现可溶表达且表达效率较低，以及难以获得具有三螺旋结构产物的问题，本发明通过优化基因序列设计及表达条件，使所述重组胶原蛋白能够在原核表达体系中以可溶形式实现高效表达，并经纯化获得目标蛋白。所述重组胶原蛋白具有良好的水溶性，并能够形成三螺旋结构。所述制备方法工艺稳定、重复性较好，适于规模化生产。本发明所述重组胶原蛋白可应用于组织工程及相关生物医用材料领域。

本发明公开了一种离体关节软骨损伤模型的构建方法及其应用，属于生物医学实验模型技术领域。以成年长白猪膝关节软骨作为实验样本，经取样、清洗后置于离体培养体系中预培养，再利用白细胞介素‑1β（IL‑1β）进行诱导处理，构建得到模拟人体早期软骨损伤及骨关节炎病理特征的离体模型。本发明明确了IL‑1β的诱导浓度及诱导时长，并建立了五维模型验证体系。相较于现有离体关节软骨损伤模型，本发明模型病理模拟度高、标准化程度强、重复性好、成本低、伦理风险小，适用范围覆盖药品、保健品、功能性食品、天然产物提取物等多类产品中软骨保护活性物质的筛选，同时可用于软骨亚健康状态及骨关节炎的机制研究，应用前景广阔。

The present invention relates to a plastic film waterproof sheet using improved point adhesive utilizing thermoplastic polymer synthetic resin films such as PP PE PVC PET EVA without adhesion for waterproofing of underground structures building walls and roofs underground passages and other concrete structures and a method for manufacturing the waterproof sheet and a waterproof construction method using the same. The construction method of the present invention includes pretreating the surface of the skin object to be waterproofed and applying a primer; heating a dissolved agent oil in a heated liquid state mixing a thermoplastic elastomer rubber compound and tackifier in a heated liquid stirrer adding aging inhibitor reinforcing agent and filler to the mixture and preparing a point adhesive applied to one side of a plastic film sheet attaching a release paper to prepare a plastic film waterproof sheet with release paper attached; removing the release paper attached to one side of the plastic film waterproof sheet and unfolding while extending so that one end overlaps on the primed surface of the skin object to be waterproofed and attaching it; attaching a reinforcing tape centered at an upper end portion of the connected plastic film waterproof sheet; and attaching a waterproof layer protective material selected from mortar plastering or concrete pouring PP PE PS foam sheets isopink PE hard boards asphalt based hard boards natural and synthetic fiber hard boards on the upper part of the waterproof sheet.

To provide an optical film capable of reducing yellowness when viewed from an oblique direction of a liquid crystal panel and maintaining heat resistance and transparency.SOLUTION: The optical film includes a resin composition and has a thickness direction retardation Rth at a wavelength of 590 nm of - 35.0 nm or more and - 5.0 nm or less. The resin composition contains an acrylic resin having a methyl methacrylate unit content of ≥98 wt.% and a triad syndiotacticity of ≥55%, and a copolymer containing a (meth)acrylonitrile unit and an aromatic vinyl unit, and has a glass transition temperature of ≥120°C. The content of the (meth)acrylonitrile unit in the copolymer is ≥22 wt.% and ≤28 wt.%. The optical film has an internal haze of 0.20% or less.SELECTED DRAWING: None

本发明公开了一株耐盐刘志恒菌及其在促进湿地芦苇生长中的应用，属于微生物技术领域。所述耐盐刘志恒菌的保藏编号为CGMCC No.38351。该菌株具备产IAA、产铁载体、解磷等多重促生功能。实验表明，该菌株可显著促进芦苇种子萌发和幼苗生长，正常条件下株高和地上部干重提高；在盐胁迫下，脯氨酸含量提高，MDA含量和Na+/K+比值降低；在盐碱与粉尘复合胁迫下，可显著提高光合效率并缓解胁迫损伤。湿地模拟验证显示，该菌株在淹水缺氧条件下第28天存活率达42.5%，促生活性显著优于对照菌株。本发明还提供了菌剂制备方案及标准化应用方法，为湿地优势植物生长以及生态修复提供了完整技术方案。

The present invention relates to a process for biorefining waste and lignocellulosic materials aimed at transforming plant waste from agriculture, agroindustrial processes, agri-food industries and the timber industry. The process comprises a first step of reactions between the lignin and the hemicellulose present in the lignocellulosic material and the SO2 in the presence of water in a heterogeneous system to three phases: solid, liquid and gaseous. The main products of these reactions are lignosulfonic acid and xylose, which are dissolved in aqueous phase. With the present invention, lignin conversions greater than 90% are achieved. The process continues with physical separation stages, lignosulfonic acid salt formation reactions, phosphate forming reaction and product purification, achieving the production of cellulose pulp, xylose, solid and dry lignosulfonic acid, alkaline, solid and dry lignosulfonates and calcium phosphate, solid and dry.